Original Articles
Acute and sub acute toxicologi
Abstract
The present investigation was intended to evaluate the toxicity of the ethanolic root extract of a traditionally used plant Saccharum spontaneum Linn. The acute toxicity studies was done on male wistar albino rats which showed no clinical signs, no mortality of the rats even under higher dosages levels (50, 150, 300, 500, 1000, 2000mg/kg b.wt) indicating the high margin of safety of the plant extract. The sub acute toxicity study was done to find out the effective dosage of the plant in rats. The varying doses(100,200,300,400, and 500mg/kg b.wt) of the plant extract were administered orally to different groups of male wistar strain of albino rats on daily basis and sacrificed after 28 days of administration. The administration of plant extract produced no significant change in organ weight and hamaetological parameters like hemoglobin, RBC, Hb, WBC, MCV, MCH, MCHC, PCV, platelet, neutrophil and lymphocyte. The record of biochemical parameters like ACP, ALP, AST, ALT, LDH, and NAG in treatment groups of rats were non significant in comparison with control group of rats. The parameters remained within the normal range. Physical, hematological parameters as well as biochemical were unaltered throughout the study. The results of study have suggested there was no obvious toxicity observed with the treatment of S.spontaneum It was found to be safe alternative for various severe infections.