Original Articles
Shantaram Nangude and Manisha
Abstract
An RP-HPLC method was developed and validated for the estimation of citrus bioflavonoids in bulk drug and solid dosage forms. The chromatographic system was equipped with Zorbax XDB C-18,250 X 4.6 mm internal diameter with 5 micron particle size column and PDA detector set at 280nm, in conjunction with a mobile phase of Methanol and Water in the ratio of 70:30 at a flow rate of 1.0 ml/min. The retention time of citrus bioflavonoids was found to be 5.602 minute. The separation was performed at ambient temperature. Linearity was observed in the concentration range of 100-1200μg/ml with correlation co-efficient 0.9999 and slope 9599.5.Percentage recovery obtained 100.07-100.62%. The percentage Assay was found to be 99.65 to 102.13%.The proposed method is precise, accurate, selective and rapid for the determination of citrus bioflavonoids in bulk drug and solid dosage forms. The proposed method is optimized and validated as per the International Conference on Harmonization (ICH) guidelines.