Research Article
K. Parameswara Rao, G.V. Raman
Abstract
A simple, accurate and sensitive RP-HPLC method stability indicating RP-HPLC method has been developed and validated for the analysis of Indinavir. The studies on selection of mobile phase and flow rate basing on peak parameters were extensively carried out. Acetonitrile, water and phosphoric acid in the ratio of 80:15:5 (v/v/v) were employed as a mobile phase in the present assay. The accuracy of the developed method was demonstrated at three concentration levels in the range of 50–150 %.The recoveries of the Indinavir from a series of spiked concentrations and the percentage recoveries were found in the range of 99.73 to 99.96 %. The % drug content of tablets obtained by the proposed method for Indinavir was found to be 99.99 %, respectively. The developed method was said to be simple, selective and accurate and is useful for the assay of Indinavir in dosage forms and can be further employed in the quality control analysis of bulk manufacturing and formulations units.